FastQCFastQC Report
Thu 2 Jun 2016
DRR028159_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028159_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621661
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG2300.044.123444145
GGTATCA16800.043.147931
TCAACGC21100.040.882154
ATCAACG21450.040.2150733
CGACGAC9250.038.399323145
GTATCAA23950.037.2279241
GTACATG46500.034.607421
CAACGCA25100.034.0782665
AACGCAG26400.031.8510136
TATCAAC28050.031.011132
TACATGG51300.030.6627852
ACATGGG51550.029.5297553
CCGATCG1306.490712E-427.8801989
GTATACG1306.490712E-427.8801981
ACGCAGA29450.027.3216467
GAGTACA35450.026.3780481
TCTCGGG18550.026.181833145
CGCAGAG29750.026.07158
ATCTACC16700.024.307526145
CATGGGG39500.024.224014
Produced by FastQC (version 0.11.5)