FastQCFastQC Report
Thu 2 Jun 2016
DRR028159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621661
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.069.777351
GTATCAA38200.050.7426761
ATCAACG41850.044.693743
TCAACGC42100.044.428344
CAACGCA44350.042.174375
AACGCAG48150.038.996536
TATCAAC48350.038.835222
GTGGTAT13000.035.1821751
ACGCAGA54400.033.849897
GTACATG48300.033.66871
CGCAGAG54450.033.4193768
TACATGG50500.030.4345572
ACATGGG52200.029.721163
GCAGAGT64150.028.0270589
TGGTATC15350.027.3931562
TAGCGCA1606.832373E-527.186534
GTCGTAC1701.0222387E-425.6228711
GTCTACG3451.07320375E-1025.2515261
CATGGGG40050.024.6183454
CTCCGAC6050.022.7734145
Produced by FastQC (version 0.11.5)