FastQCFastQC Report
Thu 2 Jun 2016
DRR028158_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028158_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2089862
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22150.046.472171
TCAACGC32350.046.384674
GTATCAA35050.046.120631
ATCAACG33250.044.9111253
CAACGCA35800.041.5096635
AACGCAG39150.038.513236
GTACATG70100.038.3649181
TACATGG70350.035.446452
ACGCAGA42150.034.2242437
TATCAAC45750.033.7494622
TGACGCG2154.8748916E-1033.7163145
CGCAGAG43750.032.309858
ACATGGG78150.031.2593063
CGACGAA6200.026.891468145
GAGTACA51550.025.7336161
GCAGAGT52950.025.3270239
CATGGGG52400.023.9327834
GTACGCG2451.9160307E-623.6702215
TACGCGC2603.21076E-622.304636
AGTACAT54000.021.8812562
Produced by FastQC (version 0.11.5)