FastQCFastQC Report
Thu 2 Jun 2016
DRR028158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2089862
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25480.12192192594534951No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24610.11775897164501771No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31150.070.162891
GTATCAA54700.055.619321
ATCAACG62950.047.5638963
TCAACGC63100.047.450834
CAACGCA65800.045.3935785
AACGCAG68750.043.6566856
TATCAAC75300.040.0527082
ACGCAGA80750.036.540547
CTCCGAC7000.036.255695145
CGCAGAG81800.036.07158
GTACATG71300.033.606511
TACATGG70700.031.994012
GCAGAGT95100.030.1882389
ACATGGG74250.029.4872043
GTGGTAT16650.029.2186131
GAGTACA56550.025.4232831
CATGGGG51550.023.6267364
AGTACAT56200.023.2203982
CAGAGTA94700.022.8899219
TGGTATC19650.022.5061282
Produced by FastQC (version 0.11.5)