FastQCFastQC Report
Thu 2 Jun 2016
DRR028157_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028157_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830575
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC15200.056.751682145
GGTATCA17050.054.42031
CGACGAA7150.053.733505145
ATCAACG25400.045.091923
TCAACGC25500.044.6308144
GTATCAA26850.043.4667241
GTACATG61000.042.06771
TACATGG63150.039.7171482
CAACGCA29950.037.9995235
AACGCAG31650.035.9584776
ACATGGG65000.035.9102023
TATCAAC33850.033.407292
ACGCAGA34650.032.6359867
CGCAGAG37050.030.1306068
CATGGGG47900.028.753684
TGACGCG2808.218194E-925.88912145
GCAGAGT46350.023.7721889
GAGTACA43750.023.3623431
CATGGGA24300.022.3733144
GTGGTAT11900.021.929611
Produced by FastQC (version 0.11.5)