FastQCFastQC Report
Thu 2 Jun 2016
DRR028157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830575
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24400.13329145213935514No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23600.1289212405938025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27500.071.273391
GTATCAA46600.058.8846661
TCAACGC56100.047.8151364
ATCAACG56350.047.731663
CAACGCA59200.045.3113065
AACGCAG61300.043.8773086
TATCAAC64450.041.9589232
ACGCAGA72200.036.751117
CGCAGAG73750.036.1753168
GTACATG62350.035.1614531
GTGGTAT15750.035.0291331
TACATGG63350.032.7308922
ACATGGG63800.031.703713
GCAGAGT84300.030.8740399
TGGTATC17600.029.2471892
ACCGATC2553.0286174E-928.4306228
GAGTACA46550.027.29071
CATGGGG48850.025.674864
CTCCGAC6850.024.346415145
CAGAGTA81200.023.8386579
Produced by FastQC (version 0.11.5)