FastQCFastQC Report
Thu 2 Jun 2016
DRR028154_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028154_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915320
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACC18400.054.75649145
TCAACGC26500.049.781064
ATCAACG27350.048.2339323
GGTATCA18350.046.215581
GTATCAA29950.045.98281
CAACGCA30300.043.537895
AACGCAG32650.040.1822286
TATCAAC36450.036.7885632
ACGCAGA36300.035.3431477
CCGACGC6250.034.792034145
GTACATG58350.034.657881
CGCAGAG36600.034.2612848
TACATGG60000.032.0135082
TGACGCG2151.816261E-830.34189145
ACATGGG63400.029.6107313
GTACGAC1555.511602E-528.0580943
GAGTACA44000.025.6986641
GCAGAGT48850.024.3342489
CATGGGG49450.023.5992494
AGTACAT46200.022.5922322
Produced by FastQC (version 0.11.5)