FastQCFastQC Report
Thu 2 Jun 2016
DRR028154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915320
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23640.12342585051061962No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22090.11533320802790134No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29200.072.3656161
GTATCAA47950.053.003131
TCAACGC53200.047.0138784
ATCAACG53400.046.7020343
CAACGCA55800.044.953195
AACGCAG59800.041.946296
TATCAAC61950.040.2575262
ACGCAGA68550.036.3805777
GTACATG57850.036.0247231
CGCAGAG69150.035.6455548
TACATGG61100.032.511672
GTGGTAT15750.031.3509331
ACATGGG62000.031.103453
TTTCGCG2151.8131686E-830.3474799
GCAGAGT83100.029.312779
CTCCGAC7100.027.578552145
CATGGGG46650.024.5541024
TGGTATC18200.023.5023142
GAGTACA43950.022.3047311
CAGAGTA81800.022.0680879
Produced by FastQC (version 0.11.5)