FastQCFastQC Report
Thu 2 Jun 2016
DRR028153_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028153_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045895
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC30550.048.8770754
ATCAACG31000.048.4013943
GGTATCA21200.045.81611
GTATCAA33900.045.7576031
CAACGCA35750.041.7676855
AACGCAG37700.039.6072856
GACGAAA9000.038.65876145
GTACATG70550.037.3984451
TATCAAC41800.036.589412
TACATGG72150.035.765382
ACGCAGA43150.034.2687727
CGCAGAG44800.032.6830448
ACATGGG79900.031.3890783
TGACGCG3351.8189894E-1228.128576145
CATGGGG59850.027.0078434
GAGTACA51800.025.4677641
GCAGAGT55400.024.7286979
GTACTAG4450.024.4332071
CATGGGA26900.021.8263914
AGTACAT56800.021.6945082
Produced by FastQC (version 0.11.5)