FastQCFastQC Report
Thu 2 Jun 2016
DRR028153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045895
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24420.11936096427236002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22780.11134491261770521No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30150.070.3033751
GTATCAA52150.055.6783451
ATCAACG57950.049.9169243
TCAACGC58400.049.532294
CAACGCA60550.047.653775
AACGCAG62350.045.929216
TATCAAC68400.042.2917632
ACGCAGA75450.037.474327
CGCAGAG77900.036.016538
GTACATG70450.035.8574031
TACATGG74450.033.4990652
ACATGGG75700.032.7535633
GCAGAGT88450.031.3107789
GTGGTAT16400.030.5411831
CATGGGG60200.026.253574
GAGTACA52550.024.864541
CTCCGAC9000.023.364588145
AGTACAT56500.022.4558032
TGGTATC19550.022.250692
CAGAGTA86500.020.6180389
Produced by FastQC (version 0.11.5)