FastQCFastQC Report
Thu 2 Jun 2016
DRR028152_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028152_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993509
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.048.700291
TGACGCG5800.044.99287145
TCAACGC27250.044.158144
GTATCAA28200.043.698751
ATCAACG28500.042.221383
CAACGCA31700.037.9592825
GTACATG62950.035.4669761
AACGCAG34300.035.2932436
TACATGG62850.034.2547152
TATCAAC36000.033.425262
ACATGGG66100.031.6931633
TCTCGGG17200.030.765474145
ACGCAGA39700.029.7622857
CTTAGGG21450.029.738876145
CGCAGAG41400.027.839798
CATGGGG53400.026.8777644
GAGTACA46850.022.7445281
ACACCGT4205.2750693E-1122.436926
GCAGAGT50900.021.9316929
ATACCGA2053.6721324E-421.216156
Produced by FastQC (version 0.11.5)