FastQCFastQC Report
Thu 2 Jun 2016
DRR028152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993509
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25270.12676140413712703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24980.12530668283915447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30850.075.545151
GTATCAA54100.058.109531
ATCAACG61550.050.294943
TCAACGC61700.050.172674
CAACGCA63900.048.672195
AACGCAG67900.045.911686
TATCAAC68900.044.8255652
ACGCAGA77200.040.099147
CGCAGAG78150.039.333398
CTCCGAC9800.035.514446145
GTACATG61600.034.415881
GCAGAGT91100.033.1054469
TACATGG63000.032.2220272
ACATGGG63550.031.1437993
GTGGTAT15400.026.8726711
CAGAGTA87750.022.9679479
CATGGGG47850.022.8780774
CGTACAC2653.7840564E-621.8861123
TGGTATC18000.021.3470942
GAGTACA45150.020.7438161
Produced by FastQC (version 0.11.5)