FastQCFastQC Report
Thu 2 Jun 2016
DRR028151_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028151_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826235
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18300.047.5314671
CGACGAC10850.047.4329145
GTATCAA26600.043.8727951
TCAACGC26550.040.679244
ATCAACG27800.039.3716163
CAACGCA30450.035.469095
GTACATG55450.034.6413961
AACGCAG32450.033.059646
TATCAAC34750.032.3316572
TACATGG57400.031.8228972
TGACGCG3650.029.788559145
ACGCAGA35850.029.5198927
ACATGGG62400.027.9951973
CGCAGAG37200.027.6691918
ATCTACC20450.027.292824145
GAGTACA42550.024.3605771
TCTCGGG18800.024.290348145
CATGGGG47450.023.8308454
GCAGAGT44650.022.5654729
AGTACAT45000.021.9067252
Produced by FastQC (version 0.11.5)