FastQCFastQC Report
Thu 2 Jun 2016
DRR028151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826235
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25850.063.751741
GTATCAA41200.051.6294061
TCAACGC45000.046.7202764
ATCAACG45150.046.0833473
CAACGCA48050.043.452925
AACGCAG50400.041.714536
TATCAAC54500.038.3103262
ACGCAGA58900.034.7099277
CTCCGAC7050.033.94684145
CGCAGAG60750.033.175578
GTACATG53800.032.385881
TACATGG57200.028.8973942
ACATGGG58250.028.003123
GCAGAGT72650.026.643769
TCGTATC2259.089472E-725.785881145
TAGATCG1701.0320426E-425.5871685
GTGGTAT15400.024.5137271
GAGTACA43550.024.3383661
CATGGGG43750.022.3704974
CCTCGCG1952.616629E-422.306769
Produced by FastQC (version 0.11.5)