FastQCFastQC Report
Thu 2 Jun 2016
DRR028150_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028150_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921852
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19550.051.166711
ATCAACG26000.042.934123
TCAACGC26450.042.477724
GTATCAA29850.039.0963941
CAACGCA30400.037.196855
AACGCAG31650.035.2697336
GTACATG60250.033.5662081
TATCAAC35500.032.4656332
TACATGG61100.030.607912
TGACGCG2651.382432E-1030.0886145
ACGCAGA36900.029.85887
CGACGAA4650.029.618006145
CGCAGAG39400.027.7802358
GAGTACA48100.027.1258031
ACATGGG66600.026.229983
AGTACAT50400.023.7305932
TCTAGCG1851.8338911E-423.5090272
AGAGTAC40450.022.937535
GCAGAGT49500.021.0868879
CAGAGTA45450.020.8926034
Produced by FastQC (version 0.11.5)