FastQCFastQC Report
Thu 2 Jun 2016
DRR028150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921852
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24410.12701290213814592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24300.12644053756480728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29300.071.3510741
GTATCAA49500.055.4322131
TCAACGC54950.049.4749344
ATCAACG55400.048.94223
CAACGCA57600.046.9470145
AACGCAG60450.044.733636
TATCAAC62400.043.4530222
ACGCAGA70200.038.210817
CGCAGAG71550.037.0845538
GTACATG59450.033.7002331
TACATGG61700.031.8432272
GCAGAGT84000.031.1565659
GTGGTAT16450.030.889281
ACATGGG65100.029.3998153
CTCCGAC7900.028.4557145
TGGTATC18400.025.2170962
TAGATCG2101.5564921E-524.1657565
CATGGGG50200.023.8287834
GTATACG2151.8491002E-523.633891
GAGTACA46050.022.0685621
Produced by FastQC (version 0.11.5)