FastQCFastQC Report
Thu 2 Jun 2016
DRR028149_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028149_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2110611
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC14700.060.159508145
TCAACGC29050.052.8994944
ATCAACG29650.051.340063
CGACGAA8700.050.824413145
GGTATCA21150.049.695771
GTATCAA35100.046.4662061
CAACGCA34350.044.5263945
AACGCAG35150.043.719216
TATCAAC38300.040.1235052
GTACATG67800.037.740431
ACGCAGA40000.037.6933867
CGCAGAG40750.036.8217548
TACATGG68950.035.744272
ACATGGG71350.031.90053
GCAGAGT51850.026.9817649
CATGGGG56900.026.1158034
GAGTACA50500.025.693511
TATAGGG8650.025.1400952
GTATAGG10800.024.1624241
TGACGCG2802.349152E-723.29948145
Produced by FastQC (version 0.11.5)