FastQCFastQC Report
Thu 2 Jun 2016
DRR028149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2110611
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24900.11797531615252645No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23700.11228975874758541No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32400.071.9202961
GTATCAA52650.052.393411
ATCAACG59800.045.3414733
TCAACGC59950.045.2280274
CAACGCA62550.043.3480455
AACGCAG66100.041.129656
TATCAAC67500.040.3849532
GTGGTAT16700.038.2523081
ACGCAGA77100.035.1675767
CGCAGAG78100.034.717298
GTACATG65850.029.8747981
GCAGAGT92400.028.4813069
TACATGG67650.027.649512
TGGTATC20200.027.6359832
ACATGGG68900.026.6211323
CTCCGAC9050.025.640072145
TAGACCG1500.001495077624.1659585
GAGTACA51550.022.9535791
CATGGGG55000.021.6175464
CAGAGTA89250.021.5259789
Produced by FastQC (version 0.11.5)