FastQCFastQC Report
Thu 2 Jun 2016
DRR028148_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028148_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2774622
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38900.045.095041
GGTATCA27450.044.0998461
TCAACGC38150.043.1314744
ATCAACG38650.042.5734943
GTACATG86100.039.9901851
TGACGCG4200.037.96963145
TACATGG90150.036.7462842
CAACGCA45050.036.5253225
TATCAAC49900.034.1373862
AACGCAG48300.033.9175346
ACATGGG95050.032.8691253
ACGCAGA52750.030.6439957
CATGGGG74200.029.2099134
CGCAGAG55050.028.7053058
GGATAGG24400.026.440105145
GAGTACA62800.025.8554061
AGTACAT66350.022.5055312
GCAGAGT67400.022.0473779
ACGAAGG13250.021.335936145
TCTCGGG34250.019.894373145
Produced by FastQC (version 0.11.5)