FastQCFastQC Report
Thu 2 Jun 2016
DRR028148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2774622
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38850.065.410941
GTATCAA62550.054.9044081
TCAACGC68800.049.209824
ATCAACG69250.049.0994263
CAACGCA73450.046.0944255
AACGCAG77150.043.8838046
TATCAAC79950.042.6189422
ACGCAGA89950.037.15557
GTACATG87150.036.9903951
CGCAGAG92750.035.6429988
TACATGG90450.034.064632
ACATGGG93750.031.7829253
GCAGAGT109750.029.9238179
CATGGGG73100.028.265124
GTGGTAT23200.026.2884311
TATCGCG1701.0321762E-425.587377
GAGTACA66600.025.0741941
TCGTATC5600.023.306564145
AGTACAT69750.022.6587332
ATGCCGC9650.021.790363145
Produced by FastQC (version 0.11.5)