FastQCFastQC Report
Thu 2 Jun 2016
DRR028147_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028147_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2349165
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC33450.045.9435164
GGTATCA24350.044.953391
ATCAACG34500.044.545233
GTATCAA35450.044.3739361
CAACGCA37250.040.8674435
AACGCAG39600.038.625296
TATCAAC44250.035.0578232
GTACATG69300.034.6241571
GAGCGAC16650.033.959774145
ACGCAGA44550.033.194567
CGCAGAG46750.031.4774028
TACATGG73050.031.258982
TGACGCG3555.456968E-1226.54602145
ACATGGG80450.025.6804873
GCAGAGT59350.023.6954779
CCGACGG5950.023.148405145
GAGTACA55250.022.3049431
CATGGGG62600.021.4230774
AGTACAT57350.020.0977822
CTGCCGC17800.019.54815145
Produced by FastQC (version 0.11.5)