FastQCFastQC Report
Thu 2 Jun 2016
DRR028147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2349165
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34850.071.255321
GTATCAA57250.054.2828061
ATCAACG65050.046.474713
TCAACGC65450.046.4122164
CAACGCA68750.044.0789765
AACGCAG71550.042.3540156
TATCAAC75750.040.2927932
ACGCAGA83950.035.838957
CGCAGAG86700.034.7021948
GTACATG69750.033.103731
GCAGAGT101000.029.358239
TACATGG75650.029.037662
ACATGGG79700.026.743423
GTGGTAT19600.026.3024561
GAGTACA57000.024.8401241
TATACCG2202.2308617E-523.0676465
TGGTATC20900.022.5473232
CAGAGTA95550.022.2313029
CATGGGG61850.021.6850284
AGTACAT61100.021.2392772
Produced by FastQC (version 0.11.5)