FastQCFastQC Report
Thu 2 Jun 2016
DRR028146_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028146_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086548
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.051.4430771
TCAACGC29500.048.16154
GTATCAA31900.047.492121
ATCAACG30000.046.8755573
GACGACC18800.044.72662145
GTACATG66000.040.30771
CAACGCA35250.040.3053675
AACGCAG38200.037.192786
TACATGG69350.036.6882172
TATCAAC40750.035.932692
ACGCAGA40700.034.7301067
CGCAGAG41600.033.1074878
ACATGGG75450.032.569153
CCGACGC8650.031.844427145
TATACCG2401.5861588E-930.2033215
CATGGGG56400.026.7331524
GAGTACA48700.025.0061191
CTACGCT1751.2577814E-424.8530184
GCAGAGT53950.024.050699
TGACGCG2704.455089E-621.477915145
Produced by FastQC (version 0.11.5)