FastQCFastQC Report
Thu 2 Jun 2016
DRR028146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086548
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22320.10697093956141915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21790.10443085900731736No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29300.067.631781
GTATCAA49800.055.241531
ATCAACG55350.049.772643
TCAACGC55700.049.5900464
CAACGCA58900.046.6496735
AACGCAG62500.044.078656
TATCAAC63350.043.6037562
ACGCAGA72050.037.93437
CGCAGAG73950.036.6655468
GTACATG61550.035.9689371
TACATGG65500.032.0997962
ACATGGG67400.031.1934093
GCAGAGT85300.030.7669379
GTGGTAT16450.026.4753171
GAGTACA46450.024.3778111
CTCCGAC7300.023.840029145
CAGAGTA83300.022.5413279
CATGGGG51950.022.328484
TCGTATC2704.4415538E-621.485458145
AGTACAT51400.020.452692
Produced by FastQC (version 0.11.5)