FastQCFastQC Report
Thu 2 Jun 2016
DRR028145_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028145_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672719
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23050.047.4838371
ATCAACG21650.046.8716133
TCAACGC21650.046.5368164
GGTATCA13800.043.595211
CAACGCA24600.041.250835
GACGAAA6700.040.02826145
GTACATG48350.039.8772241
AACGCAG27100.038.515276
TACATGG50800.037.3832782
TATCAAC30550.034.403022
ACGCAGA29800.034.05277
ACATGGG54750.033.7594873
CGCAGAG32200.030.8392988
TGACGCG2651.382432E-1030.087536145
CATGGGG37200.028.253024
GCCGACA5300.025.98469145
GAGTACA37900.023.7149541
GCAGAGT40150.021.8443749
GAGTACG3551.0181793E-720.4179151
GTCTCGC5453.6379788E-1219.9496151
Produced by FastQC (version 0.11.5)