FastQCFastQC Report
Thu 2 Jun 2016
DRR028145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672719
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21080.1260223623932053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20310.12141907875740038No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25700.074.312321
GTATCAA44650.056.1094251
ATCAACG50050.049.5388153
TCAACGC50600.049.000354
CAACGCA52800.046.9586685
AACGCAG55800.044.8237846
TATCAAC56950.043.1600042
ACGCAGA65200.038.3614547
GTACATG48850.037.6092031
CGCAGAG67400.037.324438
TACATGG48650.036.3649062
GCAGAGT75350.033.001569
ACATGGG52100.031.8655433
GTGGTAT12800.028.3660071
CATGGGG39200.026.2618854
GAGTACA37500.026.1421131
TGGTATC14550.025.4145662
AGTACAT39850.022.3795992
CAGAGTA73950.022.1561289
CATGGGA21150.018.85284
Produced by FastQC (version 0.11.5)