FastQCFastQC Report
Thu 2 Jun 2016
DRR028144_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028144_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237871
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21450.050.015531
TCAACGC31700.048.0209244
TGACGCG8250.047.447166145
GTATCAA33650.046.746071
ATCAACG32600.046.4728363
CAACGCA37500.040.0137755
AACGCAG39750.038.2959336
GTACATG72650.037.5165331
TATCAAC43550.035.1208272
TACATGG75850.033.735692
ACGCAGA45500.032.5004437
CGCAGAG47000.030.6920368
ACATGGG82450.029.8043423
GAGTACA53250.028.9954911
CTTAGGG29950.026.381538145
AGTACAT58700.024.5745432
CATGGGG62950.024.0669484
GCAGAGT58350.023.2311789
TATACAG17250.021.8516735
CATGGGA30050.020.9867534
Produced by FastQC (version 0.11.5)