FastQCFastQC Report
Thu 2 Jun 2016
DRR028144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237871
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27630.1234655616878721No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25680.11475192269795713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33150.074.034881
GTATCAA57300.057.7847821
ATCAACG65450.050.288623
TCAACGC65900.050.055234
CAACGCA69750.047.2923285
AACGCAG73300.045.0019076
TATCAAC74250.044.036552
ACGCAGA84000.039.01067
TAGACCG1502.602792E-838.6653755
GTACATG77100.038.330391
CGCAGAG85850.038.1699528
TACATGG80100.036.204342
ACATGGG84200.033.7518423
GCAGAGT100950.031.7423749
CATGGGG67050.028.3286554
GTGGTAT19050.027.8247431
GAGTACA57500.026.645131
TGGTATC19500.025.2817732
AGTACAT61000.022.8194182
CAGAGTA96850.021.7081039
Produced by FastQC (version 0.11.5)