FastQCFastQC Report
Thu 2 Jun 2016
DRR028143_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028143_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160018
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30150.046.161091
GGTATCA20200.044.856021
TCAACGC30200.044.644524
ATCAACG31350.043.006843
GTACATG66450.041.1252481
CAACGCA34550.039.2333765
TACATGG69250.037.2642482
AACGCAG37850.035.4297456
TATCAAC40250.034.5778122
CGACGAC15800.033.949764145
ACATGGG78750.031.664313
ACGCAGA42250.031.2253117
CGCAGAG41950.030.757448
CATGGGG55700.026.9387424
GAGTACA50200.024.4031091
ATCTACC22400.024.270313145
TCTCGGG23850.023.402622145
ACGATCC7800.023.233122145
GTACCGT2502.2847216E-623.1959486
GCAGAGT51450.023.105789
Produced by FastQC (version 0.11.5)