FastQCFastQC Report
Thu 2 Jun 2016
DRR028143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160018
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28400.069.537431
GTATCAA46800.052.592271
TCAACGC52350.046.1160134
ATCAACG52850.045.81693
CAACGCA55550.043.4594655
AACGCAG59550.040.6620186
TATCAAC61150.039.9556122
ACGCAGA69850.034.250887
GTACATG67450.033.6922841
CGCAGAG73100.032.6289258
TACATGG71250.031.2390522
ACATGGG72600.029.3585743
CTCCGAC8000.028.098713145
GCAGAGT85250.027.80859
TCGTATC3453.6379788E-1227.323666145
TATAACG1907.159655E-626.7109172
CATGGGG56700.025.9559654
GAGTACA52350.024.1324061
CAGAGTA81000.021.5703019
TATACAG15000.020.7826735
Produced by FastQC (version 0.11.5)