FastQCFastQC Report
Thu 2 Jun 2016
DRR028142_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028142_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441671
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25850.047.950021
GTATCAA37500.041.7518461
ATCAACG35150.041.243733
TCAACGC35150.041.037514
GTACATG82150.036.79441
CAACGCA40800.035.1769565
TACATGG84400.034.0958332
AACGCAG44550.032.0532236
TATCAAC46500.031.8002432
ACATGGG88800.031.1819743
ACGCAGA47600.029.3902727
CGCAGAG50650.027.1911378
GAGTACA58250.027.127751
TGACGCG4200.025.887804145
CATGGGG68650.025.868954
CGACGAA7600.024.79779145
AGTACAT62750.023.6806352
GCAGAGT61450.020.9967149
GTATAGA15850.020.579581
GTACTAG6050.020.3679241
Produced by FastQC (version 0.11.5)