FastQCFastQC Report
Thu 2 Jun 2016
DRR028142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441671
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35350.067.5693741
GTATCAA57600.054.0727231
ATCAACG65750.046.972453
TCAACGC65750.046.972454
CAACGCA68350.045.185645
AACGCAG71100.043.4379546
TATCAAC76750.040.33472
ACGCAGA84050.036.1414577
CGCAGAG86700.034.8695458
GTACATG81550.034.3642431
TACATGG83050.031.5135212
GCAGAGT99450.029.45149
ACATGGG86350.029.3856323
GTGGTAT17000.027.3321931
GAGTACA62100.024.5511111
CATGGGG65650.023.8532944
AGTACAT63250.022.5805782
CAGAGTA93800.022.3369759
TCGTATC3901.2201781E-820.450823145
GTATAGG9200.019.728571
Produced by FastQC (version 0.11.5)