FastQCFastQC Report
Thu 2 Jun 2016
DRR028141_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028141_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2374623
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC18900.056.379963145
GGTATCA22700.049.177241
TCAACGC31600.048.6315424
ATCAACG32650.047.0675853
GTATCAA35050.046.533291
CGACGAA10450.044.394886145
CAACGCA36150.042.510565
AACGCAG39150.039.2530446
GTACATG74100.037.369251
TATCAAC43600.037.0755542
ACGCAGA44250.034.237527
TACATGG76650.033.572642
CGCAGAG46700.032.1308868
ACATGGG79100.030.9748693
CATGGGG57500.026.2219354
GCAGAGT57200.024.9654529
TGACGCG3402.5138434E-923.45217145
GAGTACA55350.022.787721
AGAGTAC48250.021.032945
CAGAGTA53350.020.6527754
Produced by FastQC (version 0.11.5)