FastQCFastQC Report
Thu 2 Jun 2016
DRR028141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2374623
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25880.10898572110183385No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25120.10578521306329466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34350.073.1522141
GTATCAA55300.052.3993451
TCAACGC62850.045.5627824
ATCAACG63600.045.1394773
CAACGCA65800.043.4098975
AACGCAG69900.040.96746
TATCAAC76150.037.8938032
GTACATG69350.036.861611
ACGCAGA81850.034.8976367
CGCAGAG82700.034.2759638
TACATGG73850.033.576132
GTGGTAT18750.031.7607461
ACATGGG74850.031.5750683
GCAGAGT93750.029.8493239
TGGTATC20500.026.1717592
CATGGGG59050.024.186044
GAGTACA55350.023.617451
CAGAGTA92900.023.3331819
TAGACCG2202.231252E-523.0671425
AGTACAT58100.021.9629972
Produced by FastQC (version 0.11.5)