FastQCFastQC Report
Thu 2 Jun 2016
DRR028139_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028139_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3020578
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC40900.046.08144
GTATCAA45200.044.744681
ATCAACG42450.044.5695723
GGTATCA30400.040.0600361
CAACGCA47850.039.3882835
GTACATG92450.038.9695241
AACGCAG52400.036.1064576
TACATGG97450.035.779882
GAGCGAC21750.035.32826145
ACGCAGA55300.034.2129867
TATCAAC57200.033.5834662
ACATGGG103100.032.905073
CGCAGAG57400.032.2035648
CATGGGG75750.028.2302684
GAGTACA69850.027.3976381
AGTACAT74950.024.7596172
GCAGAGT72800.023.997249
TCTCGGG39050.023.946625145
TGACGCG3601.1798147E-720.135996145
CCGACGG6550.019.920803145
Produced by FastQC (version 0.11.5)