FastQCFastQC Report
Thu 2 Jun 2016
DRR028139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3020578
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40250.062.2312241
GTATCAA64400.051.182931
TCAACGC69000.046.86064
ATCAACG69950.046.224183
CAACGCA73350.044.279225
AACGCAG78750.041.3349886
TATCAAC85000.038.1250722
GTACATG95550.037.6882631
ACGCAGA89450.036.1473587
TACATGG97250.035.70822
CGCAGAG91700.035.2604268
ACATGGG104150.032.6464233
GCAGAGT110550.028.7235019
CATGGGG78350.026.8337294
GAGTACA70000.023.0255531
CAGAGTA104750.022.9776769
GTGGTAT22000.020.1308571
CTCCGAC11300.019.894249145
AGTACAT77950.019.4380282
CGCTATA3852.3899338E-718.8304772
Produced by FastQC (version 0.11.5)