FastQCFastQC Report
Thu 2 Jun 2016
DRR028138_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028138_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145058
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACC18400.051.608826145
TCAACGC26600.049.5975534
ATCAACG27300.048.060293
GTATCAA30400.046.4977041
CAACGCA30650.043.043885
GGTATCA20150.042.8097231
AACGCAG33400.039.9339146
GTACATG66000.036.903351
TATCAAC37550.035.5204472
ACGCAGA38850.034.3318567
TACATGG69050.034.22352
CGCAGAG39800.032.9659778
ACATGGG71650.031.8687363
GAGTACA48250.026.8921931
TGACGCG1958.771174E-626.021597145
CATGGGG55800.025.9816234
GCAGAGT51050.024.5652319
CCGACGC6550.024.347359145
AGTACAT53950.022.3042242
CTGCCGC16150.019.749252145
Produced by FastQC (version 0.11.5)