FastQCFastQC Report
Thu 2 Jun 2016
DRR028138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145058
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22540.10507874379154317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21810.10167557240876471No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31950.066.3525541
GTATCAA50400.056.1797331
ATCAACG56800.049.1405533
TCAACGC57050.048.9252174
CAACGCA59450.046.95015
AACGCAG63950.043.759716
TATCAAC66750.041.7078482
ACGCAGA71700.038.524197
CGCAGAG73950.036.7638328
GTACATG68600.033.125761
TACATGG70350.030.9168282
GCAGAGT87300.030.7266459
ACATGGG72150.028.9390223
CTCCGAC8700.024.170036145
GTGGTAT16950.023.558011
CATGGGG54300.023.2314934
TCGTATC2852.7781425E-722.897928145
GAGTACA52700.022.731021
CAGAGTA83100.021.5488269
AGTACAT55550.020.2295132
Produced by FastQC (version 0.11.5)