FastQCFastQC Report
Thu 2 Jun 2016
DRR028137_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028137_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2147626
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAAA8150.045.35871145
GGTATCA19900.045.1665271
TCAACGC26700.043.979664
ATCAACG28200.041.6403163
GTATCAA30100.040.456741
CAACGCA30500.038.262575
GTACATG62700.035.953481
AACGCAG33150.035.8598446
TACATGG63900.033.690212
GCCGACA6000.031.410166145
ACGCAGA36900.031.0369367
ACATGGG65350.030.9461563
TATCAAC39600.030.3851242
CGCAGAG38350.029.2964128
CATGGGG50900.027.4844884
GAGTACA45800.024.5309491
TCTCGGG23300.021.776608145
GTATAGG9350.021.7067381
TGACGCG2404.3728505E-521.141457145
AGTACAT51900.021.0890852
Produced by FastQC (version 0.11.5)