FastQCFastQC Report
Thu 2 Jun 2016
DRR028137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2147626
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31850.069.054141
GTATCAA51000.050.8106271
ATCAACG54050.047.2136153
TCAACGC55000.046.398114
CAACGCA58050.043.9603085
AACGCAG60900.041.903056
TATCAAC65850.039.6348422
ACGCAGA70500.035.6829457
CGCAGAG71250.035.205588
GTACATG61250.033.182451
TACATGG63350.030.8992232
ACATGGG62300.030.6046923
GCAGAGT85500.028.7444449
GAGTACA44800.028.1921221
GTGGTAT17300.028.1115781
CATGGGG46650.025.0204144
TAAGTCG1450.001227193724.9989815
TGGTATC19150.023.8507582
TATAACG2751.9711479E-723.7268582
AGTACAT50250.023.3727252
Produced by FastQC (version 0.11.5)