FastQCFastQC Report
Thu 2 Jun 2016
DRR028136_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028136_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246126
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36450.050.1138421
TGACGCG7250.048.990604145
GGTATCA24700.048.128421
TCAACGC36200.047.6566054
ATCAACG38000.045.208433
CAACGCA43050.040.2419935
GTACATG81900.040.0045361
AACGCAG43600.039.56816
TATCAAC45750.038.3423732
TACATGG86500.035.9497572
ACGCAGA47800.035.3331837
ACATGGG91650.032.9805833
CGCAGAG51450.032.263018
CATGGGG70500.028.7888054
CTTAGGG24400.027.924973145
GAGTACA60550.027.0550921
GCAGAGT61900.025.8795269
TCTCGGG20900.024.971285145
AGTACAT63400.024.3525852
TAGTACG2401.602235E-624.1620334
Produced by FastQC (version 0.11.5)