FastQCFastQC Report
Thu 2 Jun 2016
DRR028136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246126
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29600.13178245565921057No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29300.13044682266266452No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38250.067.1856461
GTATCAA61550.055.3158341
TCAACGC68200.048.792444
ATCAACG68500.048.578753
CAACGCA72500.045.6985475
AACGCAG75800.043.7090346
TATCAAC79100.042.435442
ACGCAGA85850.038.1700137
CGCAGAG89250.036.472238
GTACATG82900.036.2535441
TACATGG88000.033.5301862
ACATGGG89850.032.19433
GTGGTAT19450.031.3519341
GCAGAGT103450.030.4146949
GAGTACA61600.027.9300881
CATGGGG68600.027.1602154
AGTACAT67000.024.7790622
TGGTATC25850.022.4364242
CAGAGTA99500.020.8385339
CATGGGA31550.019.302084
Produced by FastQC (version 0.11.5)