FastQCFastQC Report
Thu 2 Jun 2016
DRR028134_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028134_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191625
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27000.044.5663571
GTATCAA39050.044.179291
TCAACGC38250.042.8291854
ATCAACG40200.040.751653
GTACATG84850.040.0667231
TACATGG87200.036.908752
CAACGCA44750.036.608195
AACGCAG45500.036.164076
TATCAAC46750.035.817332
ACATGGG91200.034.5746043
CGACGAA7500.030.927979145
ACGCAGA51750.030.8159247
CGCAGAG54200.029.422958
CATGGGG71550.028.6707884
GAGTACA57800.026.9633271
ACGATAG3950.025.691753145
TAGACCG1450.001228148224.9956745
GCAGAGT65700.023.1695049
AGTACAT64750.022.5018962
ATGGGGG43650.021.5884765
Produced by FastQC (version 0.11.5)