FastQCFastQC Report
Thu 2 Jun 2016
DRR028134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191625
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30900.1409912735983574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29470.1344664347230936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36550.071.1085361
GTATCAA58600.052.2806431
TCAACGC65900.045.5445254
ATCAACG67700.044.654853
CAACGCA70350.042.7666555
AACGCAG73000.041.412796
TATCAAC74550.040.3581852
GTACATG84200.039.4030841
TACATGG88550.036.2698972
ACATGGG88050.035.4046553
ACGCAGA86050.034.8795367
CGCAGAG89300.033.5289428
CATGGGG69500.030.146324
GAGTACA56550.029.2703781
GCAGAGT102300.028.4886389
CAGAGTA95800.024.5188879
AGTACAT66050.024.2577572
ATGGGGG40150.023.654135
TATAACG3508.749521E-820.7139592
GTACGAC2806.09776E-620.7134883
Produced by FastQC (version 0.11.5)