FastQCFastQC Report
Thu 2 Jun 2016
DRR028133_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028133_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464841
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC17650.064.891975145
CGACGAA8050.050.427887145
ATCAACG31250.048.713343
TCAACGC32550.046.7678034
GTATCAA34450.045.6613921
GGTATCA20750.045.4154781
CAACGCA37750.040.3256155
GTACATG73400.037.726451
AACGCAG41300.036.5083276
TATCAAC45100.034.8788262
ACGCAGA42850.034.5110367
TACATGG77000.033.2324682
CGCAGAG44450.033.1057138
ACATGGG82500.029.7868373
TGACGCG2354.3086402E-827.762163145
GAGTACA57350.025.5326921
GCAGAGT55900.025.1575649
CATGGGG56500.023.7356954
AGTACAT60250.022.7396322
TCTCGGG26650.022.304642145
Produced by FastQC (version 0.11.5)