FastQCFastQC Report
Thu 2 Jun 2016
DRR028133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2464841
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34650.068.100351
GTATCAA58500.053.243991
ATCAACG61700.050.17283
TCAACGC63000.049.022414
CAACGCA66400.046.2938585
AACGCAG70500.043.498766
TATCAAC73350.042.402512
ACGCAGA80200.037.8761027
CGCAGAG80800.037.4153948
GTACATG70900.034.920251
TACATGG70500.032.5990222
GCAGAGT95450.031.1410719
ACATGGG74800.030.5305463
CTCCGAC8350.030.39452145
GTGGTAT19600.027.412261
CATGGGG52300.026.8921594
GAGTACA56300.023.9868811
TGGTATC20800.022.6560632
ACACCGT5150.022.5236286
GTATACG3706.552E-921.5854031
Produced by FastQC (version 0.11.5)