FastQCFastQC Report
Thu 2 Jun 2016
DRR015469_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015469_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13593571
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199280.14659871199407426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158070.11628291050232496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA190250.050.848021
GTATCAA353850.041.201281
ATCAACG414250.034.5446133
TCAACGC414550.034.4427834
CAACGCA434150.032.8563965
TATCAAC445100.032.395662
AACGCAG458150.031.2121526
ACGCAGA524000.027.0119387
CGCAGAG526450.026.6082088
GTACATG486650.025.1007231
TACATGG485450.024.1635422
GCAGAGT586600.023.3516439
ACATGGG503850.022.6038173
TACCTGG111100.022.4024472
GAGTACT296150.019.95452312-13
GTACCTG132700.019.4606061
CAGAGTA569150.019.45143910-11
AGAGTAC535650.018.8674110-11
CATGGGG291150.018.5817784
GTACTTT315300.018.41779114-15
Produced by FastQC (version 0.11.5)