FastQCFastQC Report
Thu 2 Jun 2016
DRR015469_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015469_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13593571
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200880.14777573898720212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145680.10716830772429115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA171250.047.840321
GTATCAA330800.042.8481251
ATCAACG408100.034.1021583
TCAACGC410000.033.893194
CAACGCA422350.032.921345
TATCAAC438750.031.989352
AACGCAG445950.031.3338876
ACGCAGA526100.026.2751317
CGCAGAG528200.026.034378
GTACATG442450.023.7201631
GCAGAGT589900.022.8253579
TACATGG446050.022.805312
ACATGGG460200.021.1739273
AGAGTAC537150.020.0983710-11
TACCTGG108850.018.83282
GAGTACT311300.018.36697412-13
TATAACG11500.018.221782
AGTACTT327400.018.18650612-13
CAGAGTA573100.018.01980210-11
GTACCTG125350.017.3852181
Produced by FastQC (version 0.11.5)