FastQCFastQC Report
Thu 2 Jun 2016
DRR015468_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015468_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17570474
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265790.15127081944402865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204020.11611525107404616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA231350.051.0055731
GTATCAA436650.041.0005151
TCAACGC513350.034.249854
ATCAACG513250.034.2476583
CAACGCA537750.032.687335
TATCAAC560400.031.504222
AACGCAG569150.030.984996
ACGCAGA638400.027.3174577
CGCAGAG645050.026.7306358
GTACATG611850.025.1961061
TACATGG612100.024.1748222
GCAGAGT722300.023.3861339
ACATGGG633300.022.805133
GTGGTAT104100.019.3808461
CATGGGG373900.019.2524574
CAGAGTA700600.019.19493310-11
AGAGTAC668900.019.04700510-11
GAGTACT382400.018.39242612-13
GTACTTT379800.018.12883414-15
AGTACTT384100.017.06146412-13
Produced by FastQC (version 0.11.5)