FastQCFastQC Report
Thu 2 Jun 2016
DRR015468_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015468_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17570474
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264620.15060492961089153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187510.1067188056508891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA212900.049.1398741
GTATCAA416350.042.700511
TCAACGC513800.033.9590454
ATCAACG515450.033.8632853
CAACGCA531750.032.822385
TATCAAC557100.031.5604422
AACGCAG565000.030.9496366
ACGCAGA653350.026.513797
CGCAGAG658350.026.1071598
GTACATG572100.024.0636771
GCAGAGT734600.022.9141049
TACATGG576700.022.865862
ACATGGG595350.021.3845233
AGAGTAC682450.019.69687810-11
GTGGTAT102050.019.2080141
AGTACTT400400.017.92226412-13
CAGAGTA719850.017.90230610-11
GAGTACT393150.017.5467312-13
CATGGGG345400.017.1991144
GTACTTT400500.016.90616614-15
Produced by FastQC (version 0.11.5)